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Analysis of the calcium hydroxide removal of bovine root canals with different techniques by scanning electron microscopy
Abstract
Objective: To evaluate the effectiveness of calcium hydroxide removal by comparing manual files, Easy Clean and passive ultrasonic irrigation (PUI) on Ca(OH)2 removal. Method: Thirty bovine incisors teeth wereused in this study. The root canals were prepared and the samples were randomly divided into 3 groups according to the technique used for Ca(OH)2 removal (n = 10): G1: manual files; G2: Easy Clean file; G3: Passive ultrasonic irrigation. After fifteen days, specimens were sectioned and processed for observation of the middle and apical thirds by using scanning electron microscopy (SEM). One blinded and calibrated evaluator attributed scores to each specimen. The data obtained from the SEM analysis were submitted to the Kruskal-Wallys test, followed by the Dunn test. Results: There was no significant difference in Ca(OH)2 removal between manual file and Easy Clean and between Easy Clean and PUI. PUI technique significantly removed more calcium hydroxide when compared with manual file group. None of the tested techniques completely removed the Ca(OH)2 from the root canal walls. Conclusion: Regardless of the technique used, none of the protocols tested completely removed the Ca(OH)2 from the root canal. However, passive ultrasonic irrigation was more effective than manual file group.
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References
Alves FR, Siqueira JF Jr, Carmo FL, Santos AL, Peixoto RS, Rôças IN, Rosado AS. Bacterial community profiling of cryogenically ground samples from the apical and coronal root segments of teeth with apical periodontitis. J Endod. 2009;35:486-92.
Rosa GP, Nora MB, de Souza, MCA, Rangel LFGO, Chaves ESC. Medicação Intracanal Utilizada nas Clínicas de Endodontia dos Cursos de Graduação em Odontologia da Universidade Severino Sombra e na Faculdade de Odontologia de Valença. Rev Pró-Uni. 2011;2:41-52.
Soares IJ.; Goldberg F. Endodontia: técnicas e fundamentos. 2. ed. Porto Alegre: Artmed, 2011.
Zart PTM, Michelon C, Zanatta FB, Bier CAS, Manfio AP. Eficácia da irrigação ultrassônica passiva na remoção de hidróxido de cálcio. Rev Odontologia da Unesp. 2014;43:15-23.
Silva LJ, Pessoa OF, Teixeira MB, Gouveia CH, Braga RR. Micro-CT evaluation of calcium hydroxide removal through passive ultrasonic irrigation associated with or without an additional instrument. Int Endod J. 2015;48:768-73.
Alturaiki S, Lamphon H, Edrees H, Ahlquist M. Efficacy of 3 Different Irrigation Systems on Removal of Calcium Hydroxide from the Root Canal: A Scanning Electron Microscopic Study. J Endod. 2015;41:91-101.
Rodrigues CT, Duarte MAH, Guimarães BM, Vivan RR, Bernardineli N. Comparison of two methods of irrigant agitation in the removal of residual filling material in retreatment. Braz Oral Res. 2017;18:1-8.
Fraga SC, Marquizeppe GH, Duarte, MAH, Weckwerth PH, Vivan RR. Influência do veículo e do agente de irrigação na remoção de pastas de hidróxido de cálcio do canal radicular: análise em estereomicroscópio. SALUSVITA. 2012;31:119-32.
Abi-Rached GP, Herrera DR, Zaia AA, Ferraz CC, Almeida JF, Gomes BP. Efficacy of ethylene-diamine-tetra-acetic acid associated with chlorhexidine on intracanal medication removal: a scanning electron microscopy study. Microsc Res Tech. 2014; 77:735-39.
Margelos J, Eliades G, Verdelis C, Palaghias G. Interaction of calcium hydroxide with zinc oxide-eugenol type sealers: a potential clinical problem. J Endod. 1997;23:43-8.
Van der Luis LW, Versluis M, Wu MK, Wesselink PR. The evaluation of removal of calcium hydroxide paste from an artificial standardized groove in the apical root canal using different irrigation. Int Endod J. 2007;40:52-7.
Kirar DS, Jain P1, Patni P. Comparison of different irrigation and agitation methods for the removal of two types of calcium hydroxide medicaments from the root canal wall: An in-vitro study. Clujul Medical. 2017;90:327-32.
Wiseman A1, Cox TC, Paranjpe A, Flake NM, Cohenca N, Johnson JD. Efficacy of Sonic and Ultrasonic Activation for Removal of Calcium Hydroxide from Mesial Canals of Mandibular Molars: A Microtomographic Study. J Endod, 2011;37:235-8.
Kato AS, Cunha RS, da Silveira Bueno CE, Pelegrine RA, Fontana CE, de Martin AS. Investigation of the Efficacy of Passive Ultrasonic Irrigation versus Irrigation with Reciprocating Activation: An Environmental Scanning Electron Microscopic Study. J Endod. 2016;42:659-63.
Gokturk H, Ozkocak I, Buyukgebiz F, Demir O. Effectiveness of various irrigation protocols for the removal of calcium hydroxide from artificial standardized grooves. J Appl Oral Sci. 2017;25:290-8.
Guerisoli DM, Marchesan MA, Walmsley AD, Lumley PJ, Pecora JD. Evaluation of smear layer removal by EDTAC and sodium hypochlorite with ultrasonic agitation. Int Endod J 2002;35:418-21.
Gutarts R, Nusstein J, Reader A, Beck M. In vivo debridement efficacy of ultrasonic irrigation following hand-rotary instrumentation in human mandibular molars. J Endod 2005;31:166-70.
Blank-gonçalves LM, Nabeshima CK, Martins GH, Machado ME. Qualitative analysis of the removal of the smear layer in the apical third of curved roots: conventional irrigation versus activation systems. J Endod 2011;37:1268-71.
Nunes KS, Feron L, Montagner F, de Melo TAF., Kiany Scarssi et al. Analysis of root canal organic tissue dissolution capacity according to the type of irrigation solution and agitation technique. BJOS. 2016;15:70-74.
Duque JA, Duarte MA, Canali LC, Zancan RF, Vivan RR, Bernardes RA, Bramante CM.Comparative Effectiveness of New Mechanical Irrigant Agitating Devices for Debris Removal from the Canal and Isthmus of Mesial Roots of Mandibular Molars. J Endod. 2017;43:326-31.
Cesario F, Hungaro Duarte MA, Duque JA, Alcalde MP, de Andrade FB, Reis Só MV, de Vasconcelos BC, Vivan RR. Comparisons by microcomputed tomography of the efficiency of different irrigation techniques for removing dentinal debris from artificial grooves. J Conserv Dent. 2018;21:383-7.
DOI: https://doi.org/10.18256/2238-510X.2019.v8i2.3075
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